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Gene Engineering Division – DNA BANK – Shrunk Expand Primary Navigation Open Home Gene Engineering Division Top Japanese Page Top RIKEN RIKEN BioResource Research Center (BRC) Experimental Animal Division Experimental Plant Division Cell Engineering Division Microbe Division (JCM) About Gene Engineering Division Personal data protection policy Links DNA Resources Search Resources Key Word Search Browse by Depositor Gene Analysis Clone Set Resources for Gene Expression Recombinant Virus Biomass Collection Genomic DNA Microbial Genomic DNA Mouse Genomic DNA How to Order Information Order Forms Fee & Payment Terms of Use Quality control Inquiry Help Center Deposit Deposit of Resources Inquiry FAQ Technical Notes Laboratory Manual Recombinant Virus BAC Clone Microbe DNA FAQ & misc FAQ Dnaconda’s Recommendation Announcements Mail news registration Mail News Archive Lab Members Inquiry Open News News Urgent Notice: Delays in resource shipments to Europe Due to the current war in Ukraine, DNA shipments to Europe are under disruption and deliveries of DNA may experience more delays than usual. We are facing concerns that the DNA will not be delivered in favorable condition. We will determine the exact dates of transportation through close consultation with the transportation company, and will ensure that recipients are notified (with these dates) individually by email. Thank you for your understanding and cooperation. We are continuing to pray for the swift restoration of peace in Ukraine. News Monomeric Fluorescent Protein Resource, mStayGold (2023/12/18) No requirement of the Approval Forms for Venus and hKO1 BRC RESOURCE NEWS (2023/09/01) Various fluorescent proteins such as photoswitchable fluorescent protein, Kohinoor BRC RESOURCE NEWS (2023/04/28) High intensity forms of luciferase and luminescent proteins from various organisms BRC RESOURCE NEWS (2022/12/16) Split-GFP probes visualizing organelle contact sites BRC RESOURCE NEWS (2022/10/11) StayGold, highly photostable GFP SARS-CoV-2 genes and COVID-19 relevant human genes Updates (2022/05/14) New content of the NBRP: Human pathogenic virus resource (2021/09/17) Details of the NBRP Human pathogenic virus resource is now available from the following webpage. https://nbrp.jp/resource/human-pathogenic-viruses/ Notis Announcement: Revision of Distribution Fees for Bioresources in RIKEN BRC (2023/03/17) Information to Residents of the European Economic Area (EEA) Periodic security maintenance. Date & time: Our business day, 12:40 – 13:25 (JST) Dnaconda’s Recommendation Gene expression characterizing HIV-1 infected cells (2021/11/01 T.M.) Human CD4 T cells, which play a role in the control of immune system, are the primary target of HIV-1. By infection of HIV1-GFP reporter virus to humanized mouse” model in which human immune system including CD4 T cell was reconstructed, the research group discovered that characteristic factors of HIV-1-producing cells include CXCL13 gene high expression group and interferon induction gene low expression group by multidimensional analysis method. This is expected to contribute to a better understanding of the characteristics of latent HIV-1 infected cells, which are essential for the treatment of AIDS. [more…] CSII-CMV-MCS-IRES2-Bsd (cat # RDB04385), pCMV-VSV-G-RSV-Rev (cat # RDB04393), and pCAG-HIVgp (cat # RDB04394) were used to generate lentiviral vectors expressing CXCR5, a receptor for CXCL13. Research paper Aso, H. et al., Multiomics Investigation Revealing the Characteristics of HIV-1-Infected Cells In Vivo. Cell Rep. 32 (2): 107887, 2020. PMID: 32668246. Press Release University of Tokyo & AMED Multiomics investigation revealing the characteristics of HIV-1-infected cells in vivo – Clues for the development of an ‘HIV-1 cure’ – ” Jul. 14, 2020 DNA resource CSII-CMV-MCS-IRES2-Bsd (cat# RDB04385) pCMV-VSV-G-RSV-Rev (cat# RDB04393) pCAG-HIVgp (cat# RDB04394) Large-scale analysis of causative genes for severe cardiomyopathy in neonates (2021/10/26 T.M.) By analyzing the genomes of Japanese patients with severe mitochondrial cardiomyopathy in their newborn, the research group in this paper found that duplication of the ATAD3 gene cluster was involved in the onset. In addition, a large-scale analysis based on international collaborative research was conducted, and similar duplications were found in 16 families. Regarding the ATAD3 gene duplication, they indicated that abnormal fusion proteins are likely to contribute to disease development. [more…] In the analysis of abnormal ATAD3 complex formation and mitochondrial dysfunction, cell lines overexpressing ATAD3A, ATAD3C, and chimeric ATAD3A/ATAD3C genes were established using CS-CA-MCS (cat# RDB05963). Research paper Frazier, A.E. et al., Fatal perinatal mitochondrial cardiac failure caused by recurrent de novo duplications in the ATAD3 locus. Med (N Y) 2 (1): 49-73, 2021. PMID: 33575671. Press Release Chiba Children’s Hospital and others Large-scale international analysis on the causative gene of severe cardiomyopathy in newborns – Early diagnosis and development of therapies of cardiomyopathy” Jul. 10, 2020. DNA resource CS-CA-MCS (RDB05963) Discovery of agents with antiviral activity against multiple viruses (2021/10/20 T.M.) Viral infections are prevalent in the world, and the development of therapeutic drugs is an urgent issue. The research group in this paper found that 5-hydroxymethyltubercidin (HMTU) exhibited antiviral activity against multiple virus species. [more…] To investigate the effect of HMTU on the proliferation of human coronavirus in infected cells, cells that constitutively express TMPRSS2 were established based on the Vero E6 cell for SARS-CoV-2 and the A549 cell for HCoV-OC 43, respectively. CSII-CMV-MCS-IRES2-Bsd (cat # RDB04385) was used to construct a lentiviral vector expressing TMPRSS2 for cell establishment. Research paper Uemura, K. et al., 5-Hydroxymethyltubercidin exhibits potent antiviral activity against flaviviruses and coronaviruses, including SARS-CoV-2. iScience. 24 (10): 103120, 2021. PMID: 34541466. DNA resource CSII-CMV-MCS-IRES2-Bsd (cat# RDB04385) Expression and purification of recombinant galectins (2021/05/13 N.N.) Galectins belong a family of lectins and bind specifically to beta-galactosides and are known to be involved in many vital phenomena, including development, differentiation, cancer, and immunity. Dr. Nozomu Nishi of the Kagawa University has been involved in galectin research for many years and has established methods for the expression and purification of the galectin family proteins using bacteria expression system. In order to help researchers, in particular those who have limited experience in biochemical assay, he published an experimental protocol for the purification of recombinant galectin proteins consisting of 27 steps（Nishi, N., Glycoforum 23 (5), A15, 2020). This protocol, describes the operation and the handling cautions of galectin in detail. [more…] With the generosity of Dr. Nozomu Nishi, about 60 plasmids of human and about 20 plasmids of mouse and rat for the expression of galectin family proteins in E.coli were deposited in the DNA Bank. These include various useful expression plasmids of wild-type proteins, mutants in which Arg at the glycosylation site is replaced by His, and mutants in which part of the linker peptide is removed. We hope these plasmids provided by the DNA Bank will contribute to various researches of galectin-related phenomena. We are looking forward to receiving your request. Reference, Related cite Nishi, N. A note on expression and purification of recombinant galectins. Glycoforum 23 (5), A15, 2000. DNA Resource Galectin family plasmid Mitophagy visualization fluorescent sensor, mito-SRAI (2021/05/13 N.N.) Mitophagy is a selective autophagy degrading stress-damaged mitochondria. Dr. Miyawaki Atsushi and Dr. Katayama Hiroyuki of RIKEN Center for Brain Science (CBS) and Dr. Hioki Hiroyuki of Juntendo University and their...